Developing an Understanding of Brand Associations in Team Sport: Empirical Evidence from Consumers of Professional Sport
This study broadens the understanding of brand management in sport by creating the Team Association Model, a scale that identifies dimensions of brand associations, a major contributor to the creation of brand equity. Utilizing Keller’s (1993) theoretical framework of consumer-based brand equity, a thorough review of the sport literature was conducted which identified 16 potential dimensions. These 16 dimensions are derived with reference to Keller’s categorization of brand associations into ATTRIBUTE (success, head coach, star player, management, stadium, logo design, product delivery, and tradition), BENEFIT (identification, nostalgia, pride in place, escape, and peer group acceptance), and ATTITUDE (importance, knowledge, and affect). In order to evaluate the applicability of each potential dimension, a scale is developed, pre-tested, and tested on a national sample of sport consumers. Results of the confirmatory factor analysis of provided support for this paper’s theoretical notion that 16 distinct c...
The interplay between Src family kinases and receptor tyrosine kinases
Src family tyrosine kinases (SFKs) are involved in a diverse array of physiological processes, as highlighted in this review. An overview of how SFKs interact with, and participate in signaling from, receptor tyrosine kinases (RTKs) is discussed. And also, how SFKs are activated by RTKs, and how SFKs, in turn, can activate RTKs, as well as how SFKs can promote signaling from growth factor receptors in a number of ways including participation in signaling pathways required for DNA synthesis, control of receptor turnover, actin cytoskeleton rearrangements and motility, and survival are discussed.
Activation of BTK by a Phosphorylation Mechanism Initiated by SRC Family Kinases
Bruton's tyrosine kinase (BTK) is pivotal in B cell activation and development through its participation in the signaling pathways of multiple hematopoietic receptors. The mechanisms controlling BTK activation were studied here by examination of the biochemical consequences of an interaction between BTK and SRC family kinases. This interaction of BTK with SRC kinases transphosphorylated BTK on tyrosine at residue 551, which led to BTK activation. BTK then autophosphorylated at a second site. The same two sites were phosphorylated upon B cell antigen receptor cross-linking. The activated BTK was predominantly membrane-associated, which suggests that BTK integrates distinct receptor signals resulting in SRC kinase activation and BTK membrane targeting.
Profiling internet backbone traffic: behavior models and applications
Recent spates of cyber-attacks and frequent emergence of applications affecting Internet traffic dynamics have made it imperative to develop effective techniques that can extract, and make sense of, significant communication patterns from Internet traffic data for use in network operations and security management. In this paper, we present a general methodology for building comprehensive behavior profiles of Internet backbone traffic in terms of communication patterns of end-hosts and services. Relying on data mining and information-theoretic techniques, the methodology consists of significant cluster extraction, automatic behavior classification and structural modeling for in-depth interpretive analyses. We validate the methodology using data sets from the core of the Internet. The results demonstrate that it indeed can identify common traffic profiles as well as anomalous behavior patterns that are of interest to network operators and security analysts.
Scalable rate control for MPEG-4 video
This paper presents a scalable rate control (SRC) scheme based on a more accurate second-order rate-distortion model. A sliding-window method for data selection is used to mitigate the impact of a scene change. The data points for updating a model are adaptively selected such that the statistical behavior is improved. For video object (VO) shape coding, we use an adaptive threshold method to remove shape-coding artifacts for MPEG-4 applications. A dynamic bit allocation among VOs is implemented according to the coding complexities for each VO. SRC achieves more accurate bit allocation with low latency and limited buffer size. In a single framework, SRC offers multiple layers of controls for objects, frames, and macroblocks (MBs). At MB level, SRC provides finer bit rate and buffer control. At multiple VO level, SRC offers superior VO presentation for multimedia applications. The proposed SRC scheme has been adopted as part of the International Standard of the emerging ISO MPEG-4 standard.
Src homology region 2 domains direct protein-protein interactions in signal transduction.
Cytoplasmic proteins that regulate signal transduction or induce cellular transformation, including cytoplasmic protein-tyrosine kinases, p21ras GTPase-activating protein (GAP), phospholipase C gamma, and the v-crk oncoprotein, possess one or two copies of a conserved noncatalytic domain, Src homology region 2 (SH2). Here we provide direct evidence that SH2 domains can mediate the interactions of these diverse signaling proteins with a related set of phosphotyrosine ligands, including the epidermal growth factor (EGF) receptor. In src-transformed cells GAP forms heteromeric complexes, notably with a highly tyrosine phosphorylated 62-kDa protein (p62). The stable association between GAP and p62 can be specifically reconstituted in vitro by using a bacterial polypeptide containing only the N-terminal GAP SH2 domain. The efficient phosphorylation of p62 by the v-Src or v-Fps tyrosine kinases depends, in turn, on their SH2 domains and correlates with their transforming activity. In lysates of EGF-stimulated cells, the N-terminal GAP SH2 domain binds to both the EGF receptor and p62. Fusion proteins containing GAP or v-Crk SH2 domains complex with similar phosphotyrosine proteins from src-transformed or EGF-stimulated cells but with different efficiencies. SH2 sequences, therefore, form autonomous domains that direct signaling proteins, such as GAP, to bind specific phosphotyrosine-containing polypeptides. By promoting the formation of these complexes, SH2 domains are ideally suited to regulate the activation of intracellular signaling pathways by growth factors.
Analysis of a Very Large Altavista Query Log" SRC Technical note #1998-14
In this paper we present an analysis of a 280 GB AltaVista Search Engine query log consisting of approximately 1 billion entries for search requests over a period of six weeks. This represents approximately 285 million user sessions, each an attempt to fill a single information need. We present an analysis of individual queries, query duplication, and query sessions. Furthermore we present results of a correlation analysis of the log entries, studying the interaction of terms within queries. Our data supports the conjecture that web users differ significantly from the user assumed in the standard information retrieval literature. Specifically, we show that web users type in short queries, mostly look at the first 10 results only, and seldom modify the query. This suggests that traditional information retrieval techniques might not work well for answering web search requests. The correlation analysis showed that the most highly correlated items are constituents of phrases. This result indicates it may be useful for search engines to consider search terms as parts of phrases even if the user did not explicitly specify them as such.
RDFPeers: a scalable distributed RDF repository based on a structured peer-to-peer network
Centralized Resource Description Framework (RDF) repositories have limitations both in their failure tolerance and in their scalability. Existing Peer-to-Peer (P2P) RDF repositories either cannot guarantee to find query results, even if these results exist in the network, or require up-front definition of RDF schemas and designation of super peers. We present a scalable distributed RDF repository (RDFPeers) that stores each triple at three places in a multi-attribute addressable network by applying globally known hash functions to its subject predicate and object. Thus all nodes know which node is responsible for storing triple values they are looking for and both exact-match and range queries can be efficiently routed to those nodes. RDFPeers has no single point of failure nor elevated peers and does not require the prior definition of RDF schemas. Queries are guaranteed to find matched triples in the network if the triples exist. In RDFPeers both the number of neighbors per node and the number of routing hops for inserting RDF triples and for resolving most queries are logarithmic to the number of nodes in the network. We further performed experiments that show that the triple-storing load in RDFPeers differs by less than an order of magnitude between the most and the least loaded nodes for real-world RDF data.
A Survey on Energy-Efficient Routing Techniques with QoS Assurances for Wireless Multimedia Sensor Networks
The recent technological advances in micro electro-mechanical systems have promoted the development of a powerful class of sensor-based distributed intelligent systems capable of ubiquitously retrieving multimedia information, namely Wireless Multimedia Sensor Networks (WMSNs). WMSNs are gaining more popularity day by day as they are envisioned to support a large number of both non-real time and real-time multimedia applications. However, satisfying the stringent quality of service (QoS) requirements of multimedia transmission in a resource-constrained sensor network environment places new challenges to routing. As an outcome, optimal energy and application-specific QoS aware routing for WMSNs has gained considerable research attention recently. In this paper, current state-of-the-art in energy-efficient routing techniques for WMSNs is surveyed together with the highlights of the performance issues of each strategy. We outline the design challenges of routing protocols for WMSNs followed by the limitations of current techniques designed for non-multimedia data transmission. Further, a classification of recent routing protocols for WMSNs and a discussion of possible future research trends are presented.
Rapid signal transduction in living cells is a unique feature of mechanotransduction
It is widely postulated that mechanotransduction is initiated at the local force–membrane interface by inducing local conformational changes of proteins, similar to soluble ligand-induced signal transduction. However, all published reports are limited in time scale to address this fundamental issue. Using a FRET-based cytosolic Src reporter in a living cell, we quantified changes of Src activities as a local stress via activated integrins was applied. The stress induced rapid (<0.3 s) activation of Src at remote cytoplasmic sites, which depends on the cytoskeletal prestress. In contrast, there was no Src activation within 12 s of soluble epidermal growth factor (EGF) stimulation. A 1.8-Pa stress over a focal adhesion activated Src to the same extent as 0.4 ng/ml EGF at long times (minutes), and the energy levels for mechanical stimulation and chemical stimulation were comparable. The effect of both stress and EGF was less than additive. Nanometer-scale cytoskeletal deformation analyses revealed that the strong activation sites of Src by stress colocalized with large deformation sites of microtubules, suggesting that microtubules are essential structures for transmitting stresses to activate cytoplasmic proteins. These results demonstrate that rapid signal transduction via the prestressed cytoskeleton is a unique feature of mechanotransduction.
Structural Basis for the Autoinhibition of Focal Adhesion Kinase
Appropriate tyrosine kinase signaling depends on coordinated sequential coupling of protein-protein interactions with catalytic activation. Focal adhesion kinase (FAK) integrates signals from integrin and growth factor receptors to regulate cellular responses including cell adhesion, migration, and survival. Here, we describe crystal structures representing both autoinhibited and active states of FAK. The inactive structure reveals a mechanism of inhibition in which the N-terminal FERM domain directly binds the kinase domain, blocking access to the catalytic cleft and protecting the FAK activation loop from Src phosphorylation. Additionally, the FERM domain sequesters the Tyr397 autophosphorylation and Src recruitment site, which lies in the linker connecting the FERM and kinase domains. The active phosphorylated FAK kinase adopts a conformation that is immune to FERM inhibition. Our biochemical and structural analysis shows how the architecture of autoinhibited FAK orchestrates an activation sequence of FERM domain displacement, linker autophosphorylation, Src recruitment, and full catalytic activation.
C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins.
CRK protein, together with GRB2/ASH and Nck proteins, belongs to the adaptor-type Src homology (SH)2-containing molecules, which transduce signals from tyrosine kinases. Here another guanine nucleotide-releasing protein (GNRP), C3G, has been identified as a CRK SH3-binding protein. The nucleotide sequence of a 4.1-kb C3G cDNA contains a 3.2-kb open reading frame encoding a 121-kDa protein, and antibodies against C3G have been shown to detect a protein of 130-140 kDa. The carboxyl terminus of C3G has a peptide sequence homologous to GNRPs for Ras, and the expression of this carboxyl terminus region suppresses the loss of CDC25 function in the yeast Saccharomyces cerevisiae. The C3G protein expressed in Escherichia coli binds to CRK and GRB2/ASH proteins. Mutational analysis of C3G assigns the SH3 binding region to a 50-amino acid region containing a proline-rich sequence. The mRNAs of both the C3G and CRK proteins are expressed ubiquitously in human adult and fetal tissues. The results of these studies suggest that the complex of CRK and C3G, or GRB2/ASH and C3G, may transduce the signals from tyrosine kinases to Ras in a number of different tissues.
Newest findings on the oldest oncogene; how activated src does it
Oncogenic forms of the non-receptor tyrosine kinase Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that they do so by influencing the RhoA-ROCK pathway that controls contractile actin filament assembly, the STAT family of transcription factors needed for transformation, and the Cbl ubiquitin ligase that controls Src protein levels. These studies also shed light on the role of focal adhesion kinase (FAK) downstream of v-Src and other signalling pathways in controlling migration, invasion and survival of transformed cells. Src directly phosphorylates integrins and can also modulate R-Ras activity. Moreover, it stimulates the E-cadherin regulator Hakai, interacts with and phosphorylates the novel podosome-linked adaptor protein Fish, and progressively phosphorylates the gap junction component connexion 43. A recurring theme is the identification of novel and important Src substrates that mediate key biological events associated with transformation.
Size distribution, mass concentration, chemical and mineralogical composition and derived optical parameters of the boundary layer aerosol at Tinfou, Morocco, during SAMUM 2006
During the SAMUM 2006 field campaign in southern Morocco, physical and chemical properties of desert aerosols were measured. Mass concentrations ranging from 30μgm−3 for PM2.5 under desert background conditions up to 300 000μgm−3 for total suspended particles (TSP) during moderate dust storms were measured. TSP dust concentrations are correlated with the local wind speed, whereasPM10 andPM2.5 concentrations are determined by advection from distant sources. Size distributions were measured for particles with diameter between 20 nm and 500μm (parametrizations are given). Two major regimes of the size spectrum can be distinguished. For particles smaller than 500 nm diameter, the distributions show maxima around 80 nm, widely unaffected of varying meteorological and dust emission conditions. For particles larger than 500 nm, the range of variation may be up to one order of magnitude and up to three orders of magnitude for particles larger than 10μm. The mineralogical composition of aerosol bulk samples was measured by X-ray powder diffraction. Major constituents of the aerosol are quartz, potassium feldspar, plagioclase, calcite, hematite and the clay minerals illite, kaolinite and chlorite. A small temporal variability of the bulk mineralogical composition was encountered. The chemical composition of approximately 74 000 particles was determined by electron microscopic single particle analysis. Three size regimes are identified: for smaller than 500 nm in diameter, the aerosol consists of sulphates and mineral dust. For larger than 500 nm up to 50μm, mineral dust dominates, consisting mainly of silicates, and—to a lesser extent—carbonates and quartz. For diameters larger than 50μm, approximately half of the particles consist of quartz. Time series of the elemental composition show a moderate temporal variability of the major compounds. Calcium-dominated particles are enhanced during advection from a prominent dust source in Northern Africa (Chott El Djerid and surroundings). The particle aspect ratio was measured for all analysed particles. Its size dependence reflects that of the chemical composition. For larger than 500 nm particle diameter, a median aspect ratio of 1.6 is measured. Towards smaller particles, it decreases to about 1.3 (parametrizations are given). From the chemical/mineralogical composition, the aerosol complex refractive index was determined for several wavelengths from ultraviolet to near-infrared. Both real and imaginary parts show lower values for particles smaller than 500 nm in diameter (1.55–2.8 × 10−3i at 530 nm) and slightly higher values for larger particles (1.57–3.7 × 10−3i at 530 nm).
Distinct ligand preferences of Src homology 3 domains from Src, Yes, Abl, Cortactin, p53bp2, PLCgamma, Crk, and Grb2.
Src homology 3 (SH3) domains are conserved protein modules 50-70 amino acids long found in a variety of proteins with important roles in signal transduction. These domains have been shown to mediate protein-protein interactions by binding short proline-rich regions in ligand proteins. However, the ligand preferences of most SH3 domains and the role of these preferences in regulating SH3-mediated protein-protein interactions remain poorly defined. We have used a phage-displayed library of peptides of the form X6PXXPX6 to identify ligands for eight different SH3 domains. Using this approach, we have determined that each SH3 domain prefers peptide ligands with distinct sequence characteristics. Specifically, we have found that the Src SH3 domain selects peptides sharing the consensus motif LXXRPLPXpsiP, whereas Yes SH3 selects psiXXRPLPXLP, Abl SH3 selects PPXthetaXPPPpsiP, Cortactin SH3 selects +PPpsiPXKPXWL, p53bp2 SH3 selects RPXpsiPpsiR+SXP, PLCgamma SH3 selects PPVPPRPXXTL, Crk N-terminal SH3 selects psiPpsiLPpsiK, and Grb2 N-terminal SH3 selects +thetaDXPLPXLP (where psi, theta, and + represent aliphatic, aromatic, and basic residues, respectively). Furthermore, we have compared the binding of phage expressing peptides related to each consensus motif to a panel of 12 SH3 domains. Results from these experiments support the ligand preferences identified in the peptide library screen and evince the ability of SH3 domains to discern subtle differences in the primary structure of potential ligands. Finally, we have found that most known SH3-binding proteins contain proline-rich regions conforming to the ligand preferences of their respective SH3 targets.
Estrogen receptor-interacting protein that modulates its nongenomic activity-crosstalk with Src/Erk phosphorylation cascade
Numerous studies have demonstrated that estrogens induce rapid and transient activation of the Src/Erk phosphorylation cascade. Activation of this cascade triggers vital cellular functions including cell proliferation and differentiation. However, the details of the molecular mechanism of this process remain to be elucidated. We have identified a previously uncharacterized nuclear receptor-interacting protein designated as modulator of nongenomic activity of estrogen receptor (MNAR). Here we show that MNAR modulates estrogen-receptor (ER) interaction with members of the Src family of tyrosine kinases, which leads to a stimulation of Src enzymatic activity and activation of Erk1 and Erk2 kinases. We also show that MNAR, through activation of the Src/Erk phosphorylation cascade, affects ER transcriptional activity and ultimately ER-mediated gene expression. These data reveal that MNAR mediates the crosstalk between two important classes of signal transducing molecules and suggest that ER “genomic” and “nongenomic” activities are interrelated.
Yes-associated protein (YAP65) is a proline-rich phosphoprotein that binds to the SH3 domain of the Yes proto-oncogene product.
Yes belongs to the Src family of protein-tyrosine kinases. In order to understand molecular aspects of its signaling, we decided to isolate proteins that bind to the modulatory region of the Yes molecule. By generating anti-idiotypic antibodies against the aminoterminal domain of the Yes protein, we have identified, characterized and cloned a cDNA for a novel protein that binds to the Src homology domain 3 (SH3) of the Yes proto-oncogene product. The protein is of 65 kiloDalton (kDa) molecular mass, it is phosphorylated in vivo on serine and is particularly rich in proline. We named it YAP65 for Yes-Associated Protein of 65 kDa. Within the YAP65 sequence, we identified a motif, PVKQPPPLAP, similar to that found in proteins that bind to the SH3 domain of the Abl kinase. Competition assays with synthetic peptides showed the involvement of the predicted proline-rich sequence in binding between YAP65 and the Yes kinase. The YAP65 protein was also shown to bind to other signaling molecules that contain SH3 domains including Nck, Crk and Src. At lower stoichiometry, YAP65 was also shown to bind to the SH3 domains of Abl and guanosine triphosphatase activating protein (GAP). Further characterization of YAP65 should illuminate Yes signaling pathways and could also identify a novel link between protein-tyrosine and serine kinases.
An introduction to programming the meshless Element F reeGalerkin method
SummaryA detailed description of the Element Free Galerkin (EFG) method and its numerical implementation is presented with the goal of familiarizing scientists and engineers with the new computational technique. In this spirit, an in-depth explanation of the essential concepts which comprise the method is given with specific emphasis on the one-dimensional formulation. First, the EFG algorithm for a one-dimensional problem in linear elastostatics is given; the results are compared to those achievable with standard finite element techniques. A step by step explanation of the MATLAB program used to solve the problem is given with specific references to the EFG method in one-dimension. Next, a simplified two-dimensional implementation to linear elastostatics is described. Results are calculated with the method and the aid of a two-dimensional MATLAB EFG program, and conclusions are drawn about the method and its capabilities. The source programs used to solve both the one-dimensional and two-dimensional problems are provided in the Appendices and are available on the web.
The validity and reliability of GPS units for measuring distance in team sport specific running patterns.
PURPOSE To assess the validity and reliability of distance data measured by global positioning system (GPS) units sampling at 1 and 5 Hz during movement patterns common to team sports. METHODS Twenty elite Australian Football players each wearing two GPS devices (MinimaxX, Catapult, Australia) completed straight line movements (10, 20, 40 m) at various speeds (walk, jog, stride, sprint), changes of direction (COD) courses of two different frequencies (gradual and tight), and a team sport running simulation circuit. Position and speed data were collected by the GPS devices at 1 and 5 Hz. Distance validity was assessed using the standard error of the estimate (±90% confidence intervals [CI]). Reliability was estimated using typical error (TE) ± 90% CI (expressed as coefficient of variation [CV]). RESULTS Measurement accuracy decreased as speed of locomotion increased in both straight line and the COD courses. Difference between criterion and GPS measured distance ranged from 9.0% to 32.4%. A higher sampling rate improved validity regardless of distance and locomotion in the straight line, COD and simulated running circuit trials. The reliability improved as distance traveled increased but decreased as speed increased. Total distance over the simulated running circuit exhibited the lowest variation (CV 3.6%) while sprinting over 10 m demonstrated the highest (CV 77.2% at 1 Hz). CONCLUSION Current GPS systems maybe limited for assessment of short, high speed straight line running and efforts involving change of direction. An increased sample rate improves validity and reliability of GPS devices.
Physical Layer Security for Next Generation Wireless Networks: Theories, Technologies, and Challenges
Physical layer security (PHY-security) takes the advantages of channel randomness nature of transmission media to achieve communication confidentiality and authentication. Wiretap coding and signal processing technologies are expected to play vital roles in this new security mechanism. PHY-security has attracted a lot of attention due to its unique features and the fact that our daily life relies heavily on wireless communications for sensitive and private information transmissions. Compared to conventional cryptography that works to ensure all involved entities to load proper and authenticated cryptographic information, PHY-security technologies perform security functions without considering about how those security protocols are executed. In other words, it does not require to implement any extra security schemes or algorithms on other layers above the physical layer. This survey introduces the fundamental theories of PHY-security, covering confidentiality and authentication, and provides an overview on the state-of-the-art works on PHY-security technologies that can provide secure communications in wireless systems, along with the discussions on challenges and their proposed solutions. Furthermore, at the end of this paper, the open issues are identified as our future research directions.
sensor network wireless sensor network wireless sensor power plant wireless network face recognition breast cancer wireless lan sensor datum thermal power sparse representation systematic review wireless system thermal power plant amino acid solar thermal wireless multimedia cancer cell multimedia sensor network wireless multimedia sensor multimedia sensor multi-user mimo generation wireles based classification transcription factor representation based cell line behavior model generation wireless network solar thermal power behavior modeling based face recognition sparse representation based tumor cell receiver system robust face recognition amino acid sequence frog leaping algorithm frog leaping theory of planned growth factor signal transduction leaping algorithm scalable distributed planned behavior collaborative representation central receiver suspended sediment optical parameter team sport face recognition problem bone marrow protein domain generation wireless system cell growth representation based classification peripheral blood sparse representation-based cell proliferation professional sport protein kinase short rotation sparse representation-based classification revenue sharing signal transduction pathway cell adhesion myeloid leukemia central receiver system sparse coding model body tissue phage display generation wireless lan growth factor receptor sports league cell nucleu tyrosine kinase tumor progression cell activation solar thermal central competitive balance thermal central receiver human colon cell invasion real-world face recognition sparse representation-based classifier professional team biological signaling enhancing feature protein tyrosine scalar sensor datum focal adhesion protein protein interaction activation action dna, complementary interleukin receptor common gamma subunit adaptor proteins, signal transducing 1-phosphatidylinositol 3-kinase immunologic deficiency syndrome blood platelet sh3 domain myeloid leukemia, chronic protein tyrosine phosphatase erbb receptor fyn gene abl oncogene tyrosine phosphorylation proline-rich domain src gene tyrosine kinase domain src-family kinase v-src protein intracellular signal transduction lyn gene review [publication type] transcription, genetic homologous gene ligand binding domain tissue membrane staphylococcal protein a leukemia, b-cell kinase activity protein tyrosine kinase united state